Real-Time PCR Data Sets by Boggy et al. (2010)

Real-time quantitative polymerase chain reaction (qPCR) data sets by Boggy et al. (2008) . This package provides a dilution series for one PCR target: a random sequence that minimizes secondary structure and off-target primer binding. The data set is a six-point, ten-fold dilution series. For each concentration there are two replicates. Each amplification curve is 40 cycles long. Original raw data file: < https://journals.plos.org/plosone/article/file?type=supplementary&id=10.1371/journal.pone.0012355.s004>.

boggy

{boggy} provides the real-time PCR data set s1 by Boggy et al. (2010) in tidy format.

Installation

install.packages("boggy")

Usage

library(boggy)
library(ggplot2)

s1 |>
  ggplot(mapping = aes(
    x = cycle,
    y = fluor,
    group = well,
    col = format(copies, big.mark = ",", scientific = FALSE)
  )) +
  geom_line(linewidth = 0.2) +
  geom_point(size = 0.2) +
  labs(color = "Copy number")

References

Gregory J. Boggy & Peter J. Woolf. A Mechanistic Model of PCR for Accurate Quantification of Quantitative PCR Data. PLoS ONE 5:8 (2010). doi: 10.1371/journal.pone.0012355.