We provide the monthly number of HIV and antiretroviral therapy (ART) cases of male, female, children and transgender as well as for the whole of Pakistan reported at various treatment centers in Pakistan from January 2016 to December 2021. Related works include: a) Imran, M., Nasir, J. A., & Riaz, S. (2018). Regional pattern of HIV cases in Pakistan. Journal of Postgraduate Medical Institute, 32(1), 9-13. < https://jpmi.org.pk/index.php/jpmi/article/view/2108>.