Analysis of Surface Plasmon Resonance Data

Analysis of Surface Plasmon Resonance (SPR) and Biolayer Interferometry data, with automations for high-throughput SPR. This version of the package fits the 1: 1 binding model, with and without bulkshift. It offers optional local or global Rmax fitting. The user must provide a sample sheet and a Carterra output file in Carterra's current format. There is a utility function to convert from Carterra's old output format. The user may run a custom pipeline or use the provided 'Runscript', which will produce a pdf file containing fitted Rmax, ka, kd and standard errors, a plot of the sensorgram and fits, and a plot of residuals. The script will also produce a .csv file with all of the relevant parameters for each spot on the SPR chip.

High-Throughput SPR Kinetics Analysis Software

This R package provides functions and a pipeline to analyze SPR data. It takes as input the sample information and the Carterra time series and produces a pdf with sensorgrams, fits, rate constants, Rmax, plots of residuals and standard errors of parameter estimates. It also produces a .csv file with the relevant parameters and standard errors.

Current features include:

1:1 binding model
Options for local or global Rmax fitting
Automated concentration range determination
Automated dissociation window approximation
Automated bulkshift determination

The motivations for developing this package are

to provide an open-source tool for analyzing high-throughput SPR kinetic data
to automate some of the steps in analysis, such as determining the length of dissociation window and concentration range to include in fits and whether or not to include bulk-shift in fitting.
to provide a platform for further development of multivalent binding kinetics models, such as bivalent analyte, parallel binding and more. These models are currently not an option in commercial software when using non-regenerative SPR data.